Isolation and Characterization of <i>Striga hermonthica</i> Seed-Bank Depleting Bacteria from <i>Striga</i> Infested Sorghum Growing Areas of Ethiopia

Getachew Yilma; Mamo Bekele; Fasil Assefa; Taye Tessema

doi:doi:10.11648/j.ijfsb.20240904.11

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Isolation and Characterization of Striga hermonthica Seed-Bank Depleting Bacteria from Striga Infested Sorghum Growing Areas of Ethiopia

Getachew Yilma^*

, Mamo Bekele, Fasil Assefa, Taye Tessema

Published in International Journal of Food Science and Biotechnology (Volume 9, Issue 4)

Received: 3 September 2024 Accepted: 29 September 2024 Published: 13 November 2024

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Abstract

Striga hermonthica is a very serios parasitic weed of (Sorghum bicolor L. Moench). It attack host plants by living as hemi-parasite and attaching small sucker root system to host plant. A study was undertaken to evaluate the effect of bacterial isolate obtained from soil samples collected from Striga infested sorghum growing fields in Amhara, Tigray and Oromia Regions to inhibit (deplete) the Striga seed bank. Surface sterilized and conditioned Striga seeds were buried in the soil by using teabag to determine germinated, viable and decayed seeds at different times after burial. A total of 44 isolates were isolated from germinated and decayed striga seeds and tested for their effect on germination and seed decay of Striga seeds. Six isolates (GS29, GS32, GS34, GS39, GS42 and GS45) were stimulated 10.33, 9.0, 9.67, 8.33, 5.33 and 8.33 Striga seeds germination from the total of 35 seeds respectively in the absence of host plant synthetic stimulant. On the other hand four isolates (SD3, SD9, SD36 and SD46) significantly decayed Striga seeds at P<0.05 (21, 20, 21.33 and 21 seeds respectively) in order to reduce Striga attack. Selected isolates were characterized by using biochemical tests, and three isolates were classified under the genus Pseudomonas while the other three isolates were grouped under the genus Klebsiella. The four isolates that showed seed decay were classified under the genus Bacillus morphologically.

Published in	International Journal of Food Science and Biotechnology (Volume 9, Issue 4)
DOI	10.11648/j.ijfsb.20240904.11
Page(s)	58-68
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Striga Hermonthica, Germination, Stimulant, Seed-Decay, Viability, Parasitic-Weed

References

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APA Style

Yilma, G., Bekele, M., Assefa, F., Tessema, T. (2024). Isolation and Characterization of Striga hermonthica Seed-Bank Depleting Bacteria from Striga Infested Sorghum Growing Areas of Ethiopia. International Journal of Food Science and Biotechnology, 9(4), 58-68. https://doi.org/10.11648/j.ijfsb.20240904.11

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ACS Style

Yilma, G.; Bekele, M.; Assefa, F.; Tessema, T. Isolation and Characterization of Striga hermonthica Seed-Bank Depleting Bacteria from Striga Infested Sorghum Growing Areas of Ethiopia. Int. J. Food Sci. Biotechnol. 2024, 9(4), 58-68. doi: 10.11648/j.ijfsb.20240904.11

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AMA Style

Yilma G, Bekele M, Assefa F, Tessema T. Isolation and Characterization of Striga hermonthica Seed-Bank Depleting Bacteria from Striga Infested Sorghum Growing Areas of Ethiopia. Int J Food Sci Biotechnol. 2024;9(4):58-68. doi: 10.11648/j.ijfsb.20240904.11

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@article{10.11648/j.ijfsb.20240904.11,
  author = {Getachew Yilma and Mamo Bekele and Fasil Assefa and Taye Tessema},
  title = {Isolation and Characterization of Striga hermonthica Seed-Bank Depleting Bacteria from Striga Infested Sorghum Growing Areas of Ethiopia
},
  journal = {International Journal of Food Science and Biotechnology},
  volume = {9},
  number = {4},
  pages = {58-68},
  doi = {10.11648/j.ijfsb.20240904.11},
  url = {https://doi.org/10.11648/j.ijfsb.20240904.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijfsb.20240904.11},
  abstract = {Striga hermonthica is a very serios parasitic weed of (Sorghum bicolor L. Moench). It attack host plants by living as hemi-parasite and attaching small sucker root system to host plant. A study was undertaken to evaluate the effect of bacterial isolate obtained from soil samples collected from Striga infested sorghum growing fields in Amhara, Tigray and Oromia Regions to inhibit (deplete) the Striga seed bank. Surface sterilized and conditioned Striga seeds were buried in the soil by using teabag to determine germinated, viable and decayed seeds at different times after burial. A total of 44 isolates were isolated from germinated and decayed striga seeds and tested for their effect on germination and seed decay of Striga seeds. Six isolates (GS29, GS32, GS34, GS39, GS42 and GS45) were stimulated 10.33, 9.0, 9.67, 8.33, 5.33 and 8.33 Striga seeds germination from the total of 35 seeds respectively in the absence of host plant synthetic stimulant. On the other hand four isolates (SD3, SD9, SD36 and SD46) significantly decayed Striga seeds at PStriga attack. Selected isolates were characterized by using biochemical tests, and three isolates were classified under the genus Pseudomonas while the other three isolates were grouped under the genus Klebsiella. The four isolates that showed seed decay were classified under the genus Bacillus morphologically.
},
 year = {2024}
}

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TY  - JOUR
T1  - Isolation and Characterization of Striga hermonthica Seed-Bank Depleting Bacteria from Striga Infested Sorghum Growing Areas of Ethiopia

AU  - Getachew Yilma
AU  - Mamo Bekele
AU  - Fasil Assefa
AU  - Taye Tessema
Y1  - 2024/11/13
PY  - 2024
N1  - https://doi.org/10.11648/j.ijfsb.20240904.11
DO  - 10.11648/j.ijfsb.20240904.11
T2  - International Journal of Food Science and Biotechnology
JF  - International Journal of Food Science and Biotechnology
JO  - International Journal of Food Science and Biotechnology
SP  - 58
EP  - 68
PB  - Science Publishing Group
SN  - 2578-9643
UR  - https://doi.org/10.11648/j.ijfsb.20240904.11
AB  - Striga hermonthica is a very serios parasitic weed of (Sorghum bicolor L. Moench). It attack host plants by living as hemi-parasite and attaching small sucker root system to host plant. A study was undertaken to evaluate the effect of bacterial isolate obtained from soil samples collected from Striga infested sorghum growing fields in Amhara, Tigray and Oromia Regions to inhibit (deplete) the Striga seed bank. Surface sterilized and conditioned Striga seeds were buried in the soil by using teabag to determine germinated, viable and decayed seeds at different times after burial. A total of 44 isolates were isolated from germinated and decayed striga seeds and tested for their effect on germination and seed decay of Striga seeds. Six isolates (GS29, GS32, GS34, GS39, GS42 and GS45) were stimulated 10.33, 9.0, 9.67, 8.33, 5.33 and 8.33 Striga seeds germination from the total of 35 seeds respectively in the absence of host plant synthetic stimulant. On the other hand four isolates (SD3, SD9, SD36 and SD46) significantly decayed Striga seeds at PStriga attack. Selected isolates were characterized by using biochemical tests, and three isolates were classified under the genus Pseudomonas while the other three isolates were grouped under the genus Klebsiella. The four isolates that showed seed decay were classified under the genus Bacillus morphologically.

VL  - 9
IS  - 4
ER  -

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Author Information

Getachew Yilma

Ethiopian Institute of Agricultural Research, Fogera National Rice Research and Training Center, Woreta, Ethiopia

Contact Email

http://orcid.org/0009-0003-4738-6940
Mamo Bekele

Ethiopian Institute of Agricultural Research, Holeta National Agricultural Biotechnology Research Center, Holeta Town, Ethiopia

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Fasil Assefa

Department of Microbial Cellular and Molecular Biology, Addis Ababa University, Addis Ababa, Ethiopia

Contact Email
Taye Tessema

Ethiopian Institute of Agricultural Research National Weed Research Program, Addis Ababa, Ethiopia

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APA Style

Yilma, G., Bekele, M., Assefa, F., Tessema, T. (2024). Isolation and Characterization of Striga hermonthica Seed-Bank Depleting Bacteria from Striga Infested Sorghum Growing Areas of Ethiopia. International Journal of Food Science and Biotechnology, 9(4), 58-68. https://doi.org/10.11648/j.ijfsb.20240904.11

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ACS Style

Yilma, G.; Bekele, M.; Assefa, F.; Tessema, T. Isolation and Characterization of Striga hermonthica Seed-Bank Depleting Bacteria from Striga Infested Sorghum Growing Areas of Ethiopia. Int. J. Food Sci. Biotechnol. 2024, 9(4), 58-68. doi: 10.11648/j.ijfsb.20240904.11

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AMA Style

Yilma G, Bekele M, Assefa F, Tessema T. Isolation and Characterization of Striga hermonthica Seed-Bank Depleting Bacteria from Striga Infested Sorghum Growing Areas of Ethiopia. Int J Food Sci Biotechnol. 2024;9(4):58-68. doi: 10.11648/j.ijfsb.20240904.11

Copy | Download

@article{10.11648/j.ijfsb.20240904.11,
  author = {Getachew Yilma and Mamo Bekele and Fasil Assefa and Taye Tessema},
  title = {Isolation and Characterization of Striga hermonthica Seed-Bank Depleting Bacteria from Striga Infested Sorghum Growing Areas of Ethiopia
},
  journal = {International Journal of Food Science and Biotechnology},
  volume = {9},
  number = {4},
  pages = {58-68},
  doi = {10.11648/j.ijfsb.20240904.11},
  url = {https://doi.org/10.11648/j.ijfsb.20240904.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijfsb.20240904.11},
  abstract = {Striga hermonthica is a very serios parasitic weed of (Sorghum bicolor L. Moench). It attack host plants by living as hemi-parasite and attaching small sucker root system to host plant. A study was undertaken to evaluate the effect of bacterial isolate obtained from soil samples collected from Striga infested sorghum growing fields in Amhara, Tigray and Oromia Regions to inhibit (deplete) the Striga seed bank. Surface sterilized and conditioned Striga seeds were buried in the soil by using teabag to determine germinated, viable and decayed seeds at different times after burial. A total of 44 isolates were isolated from germinated and decayed striga seeds and tested for their effect on germination and seed decay of Striga seeds. Six isolates (GS29, GS32, GS34, GS39, GS42 and GS45) were stimulated 10.33, 9.0, 9.67, 8.33, 5.33 and 8.33 Striga seeds germination from the total of 35 seeds respectively in the absence of host plant synthetic stimulant. On the other hand four isolates (SD3, SD9, SD36 and SD46) significantly decayed Striga seeds at PStriga attack. Selected isolates were characterized by using biochemical tests, and three isolates were classified under the genus Pseudomonas while the other three isolates were grouped under the genus Klebsiella. The four isolates that showed seed decay were classified under the genus Bacillus morphologically.
},
 year = {2024}
}

Copy | Download

TY  - JOUR
T1  - Isolation and Characterization of Striga hermonthica Seed-Bank Depleting Bacteria from Striga Infested Sorghum Growing Areas of Ethiopia

AU  - Getachew Yilma
AU  - Mamo Bekele
AU  - Fasil Assefa
AU  - Taye Tessema
Y1  - 2024/11/13
PY  - 2024
N1  - https://doi.org/10.11648/j.ijfsb.20240904.11
DO  - 10.11648/j.ijfsb.20240904.11
T2  - International Journal of Food Science and Biotechnology
JF  - International Journal of Food Science and Biotechnology
JO  - International Journal of Food Science and Biotechnology
SP  - 58
EP  - 68
PB  - Science Publishing Group
SN  - 2578-9643
UR  - https://doi.org/10.11648/j.ijfsb.20240904.11
AB  - Striga hermonthica is a very serios parasitic weed of (Sorghum bicolor L. Moench). It attack host plants by living as hemi-parasite and attaching small sucker root system to host plant. A study was undertaken to evaluate the effect of bacterial isolate obtained from soil samples collected from Striga infested sorghum growing fields in Amhara, Tigray and Oromia Regions to inhibit (deplete) the Striga seed bank. Surface sterilized and conditioned Striga seeds were buried in the soil by using teabag to determine germinated, viable and decayed seeds at different times after burial. A total of 44 isolates were isolated from germinated and decayed striga seeds and tested for their effect on germination and seed decay of Striga seeds. Six isolates (GS29, GS32, GS34, GS39, GS42 and GS45) were stimulated 10.33, 9.0, 9.67, 8.33, 5.33 and 8.33 Striga seeds germination from the total of 35 seeds respectively in the absence of host plant synthetic stimulant. On the other hand four isolates (SD3, SD9, SD36 and SD46) significantly decayed Striga seeds at PStriga attack. Selected isolates were characterized by using biochemical tests, and three isolates were classified under the genus Pseudomonas while the other three isolates were grouped under the genus Klebsiella. The four isolates that showed seed decay were classified under the genus Bacillus morphologically.

VL  - 9
IS  - 4
ER  -

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